| Field | Specification |
|---|---|
| Target | |
| Alternative names | P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD62P; Selp; Grmp |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse CD62P recombinant protein (Position: W42-A267). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets P-selectin (gene Selp) in mouse and rat samples and is validated for ELISA, flow cytometry and Western blot. The predicted molecular weight is 83.1 kDa, and the supplier reports an observed band at about 110 kDa.
P-selectin (SELP, CD62P) is an adhesion molecule stored in platelet α-granules and endothelial Weibel-Palade bodies and moved to the cell surface upon activation. It binds PSGL-1 on leukocytes to start their rolling during inflammation and links platelets with leukocytes, and it is widely used as a platelet activation marker.
| Target | P-selectin (gene Selp; UniProt Q01102, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 83.1 kDa |
| Observed MW | 110 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells ELISA (0.1-0.5μg/ml) |
| ELISA | Not specified by the supplier |
Samples with a confirmed band (WB): mouse heart tissue, rat heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with a confirmed signal (flow cytometry): RAW264.7 cells.
Immunogen
Recombinant mouse CD62P fragment (Trp42–Ala267), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places P-selectin in the cell membrane. Tissue expression noted by UniProt (mouse): Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Fengming Zhang et al. (2012) Fabrication of biomolecule-PEG micropattern on titanium surface and its effects on platelet adhesion. Colloids and Surfaces B-Biointerfaces. 10.1016/j.colsurfb.2012.02.018
Huafeng Yang et al. (2024) Cornus Officinalis Total Glycosides Alleviate Granulomatous Lobular Mastitis via the B7-CD28/CTLA-4 Costimulatory Pathway. Chemistry & Biodiversity. 10.1002/cbdv.202401539
Rui Zhang et al. (2020) Irisin Pretreatment Protects Kidneys against Acute Kidney Injury Induced by Ischemia/Reperfusion via Upregulating the Expression of Uncoupling Protein 2. Biomed Research International. 10.1155/2020/6537371
