| Field | Specification |
|---|---|
| Target | |
| Alternative names | CD63 antigen; CD antigen CD63; Cd63 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Cd63 recombinant protein (Position: A103-I203). Mouse Cd63 shares 67.3% and 93.1% amino acid (aa) sequence identity with human and rat Cd63, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects CD63 antigen in mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 25.8 kDa, and the supplier reports an observed band at about 60 kDa.
CD63 is a tetraspanin found in late endosomes, lysosomes and multivesicular bodies, and on the cell surface. It is enriched on exosomes, where it is one of the most widely used markers, and it also serves as a marker of basophil and platelet activation.
| Target | CD63 antigen (gene Cd63; UniProt P41731, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 25.8 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat lung tissue, rat PC-12 cells, rat RH35 cells, mouse lung tissue, mouse RAW264.7 cells, mouse ANA-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant mouse Cd63 fragment (Ala103–Ile203), expressed in E. coli. Sequence identity with the human and rat orthologs: 67.3% and 93.1%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places CD63 antigen in the cell membrane, lysosome membrane, late endosome membrane and endosome. Tissue expression noted by UniProt (mouse): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yushan Wang et al. (2026) NsPEFs-enriched ADSCs-EVs alleviate osteoarthritis via RSPO3-mediated dual pro-chondrogenic and pro-M2 macrophage properties. Bioactive Materials. 10.1016/j.bioactmat.2026.01.006
Jingwei Yu et al. (2023) Serum exosomes derived from spontaneously hypertensive rats induce cardiac hypertrophy in vitro and in vivo by increasing autocrine release of angiotensin II in cardiomyocytes. Biochemical Pharmacology. 10.1016/j.bcp.2023.115462
Heng Zhou et al. (2025) Multifunctional copper-based metal-organic frameworks/chitosan derivative/exosome composite sponge for skin healing. iScience. 10.1016/j.isci.2025.114176
