| Field | Specification |
|---|---|
| Target | |
| Alternative names | 5'-nucleotidase; 5'-NT; 5'-deoxynucleotidase; Ecto-5'-nucleotidase; IMP-specific 5'-nucleotidase; Thymidylate 5'-phosphatase; CD antigen CD73; Nt5e; Nt5; Nte |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse CD73/Nt5e recombinant protein (Position: S337-S551). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes 5'-nucleotidase (gene Nt5e) in mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 63.9 kDa, and the supplier reports an observed band at about 63 kDa.
CD73 (NT5E, ecto-5′-nucleotidase) is a GPI-anchored enzyme on the outer surface of many cell types that converts extracellular AMP into adenosine and phosphate. The adenosine it generates signals through adenosine receptors to dampen inflammation and T-cell activity, so CD73 is widely studied in tumor immunology, vascular biology and purinergic signaling.
| Target | 5'-nucleotidase (gene Nt5e; UniProt Q61503, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 63.9 kDa |
| Observed MW | 63 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat PC-12 cells, rat C6 cells, rat RH35 cells, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): mouse spleen tissue.
Immunogen
Recombinant mouse CD73/Nt5e fragment (Ser337–Ser551), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places 5'-nucleotidase in the cell membrane. Tissue expression noted by UniProt (mouse): Expressed at high levels in the placenta, kidney, lung and stomach and at lower levels in the thymus, spleen, skeletal muscle and esophagus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Pengfei Liu et al. (2014) Administration of BMSCs with Muscone in Rats with Gentamicin-Induced AKI Improves Their Therapeutic Efficacy. PLoS One. 10.1371/journal.pone.0097123
RuiYin Lai et al. (2026) Effects of intermittent hypoxia on adipose-derived mesenchymal stem cells in protecting alveolar type II cells from injury. PLoS One. 10.1371/journal.pone.0340291
Manna Camelia et al. (2023) Canine umbilical cord tissue derived mesenchymal stem cells naturally express mRNAs of some antimicrobial peptides. Veterinary Research Communications. 10.1007/s11259-023-10098-x
