| Field | Specification |
|---|---|
| Target | |
| Alternative names | 5'-nucleotidase;5'-NT;3.1.3.5;Ecto-5'-nucleotidase;CD73;NT5E;NT5, NTE; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD73 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABOA-14 is a rabbit monoclonal antibody that recognizes 5'-nucleotidase (gene NT5E) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 63.4 kDa, and the supplier reports an observed band at about 70 kDa.
CD73 (NT5E, ecto-5′-nucleotidase) is a GPI-anchored enzyme on the outer surface of many cell types that converts extracellular AMP into adenosine and phosphate. The adenosine it generates signals through adenosine receptors to dampen inflammation and T-cell activity, so CD73 is widely studied in tumor immunology, vascular biology and purinergic signaling.
| Target | 5'-nucleotidase (gene NT5E; UniProt P21589, human) |
|---|---|
| Clone | ABOA-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 63.4 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): A375 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CD73.
Reactivity Notes
The supplier lists reactivity with human. UniProt places 5'-nucleotidase in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
