| Field | Specification |
|---|---|
| Target | |
| Alternative names | HLA class II histocompatibility antigen gamma chain;HLA-DR antigens-associated invariant chain;Ia antigen-associated invariant chain;Ii;p33;CD74;CD74;DHLAG; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD74 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOIF-3 is a rabbit monoclonal antibody that recognizes HLA class II histocompatibility antigen gamma chain (gene CD74) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 33.5 kDa, and the supplier reports an observed band at about 34 kDa.
CD74, the MHC class II invariant chain, binds newly made class II α/β heterodimers in the endoplasmic reticulum and guides them to endosomal compartments for peptide loading. It is also a cell surface receptor for macrophage migration inhibitory factor (MIF), linking it to cell survival and inflammatory signaling.
| Target | HLA class II histocompatibility antigen gamma chain (gene CD74; UniProt P04233, human) |
|---|---|
| Clone | AOIF-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 33.5 kDa |
| Observed MW | 34 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human Daudi cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human testis cancer tissue, human prostate cancer tissue, human liver cancer tissue, human lung cancer tissue, human colon cancer tissue, human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human CD74.
Reactivity Notes
The supplier lists reactivity with human. UniProt places HLA class II histocompatibility antigen gamma chain in the cell membrane, endoplasmic reticulum membrane, Golgi apparatus and endosome. Tissue expression noted by UniProt (human): Detected in urine (at protein level)... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
