| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-cell surface glycoprotein CD8 alpha chain; CD8 antigen 32 kDa chain; OX-8 membrane antigen; CD8a |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse CD8 recombinant protein (Position: K39-K245). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets T-cell surface glycoprotein CD8 alpha chain (gene Cd8a) in mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 27.5 kDa, and the supplier reports an observed band at about 26 kDa.
CD8α (CD8A) is a transmembrane glycoprotein that forms the CD8 co-receptor, usually as a CD8αβ heterodimer on cytotoxic T cells and as a CD8αα homodimer on some other lymphocytes. It binds MHC class I and recruits LCK to enhance T-cell receptor signaling, and it is the standard marker for cytotoxic T cells.
| Target | T-cell surface glycoprotein CD8 alpha chain (gene Cd8a; UniProt P01731, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 27.5 kDa |
| Observed MW | 26 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat thymus tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue, rat spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): mouse spleen tissue.
Immunogen
Recombinant mouse CD8 fragment (Lys39–Lys245), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places T-cell surface glycoprotein CD8 alpha chain in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yidi Qu et al. (2023) The involvement of Th1 cell differentiation in the anti-tumor effect of purified polysaccharide from Sanghuangporus vaninii in colorectal cancer via multi-omics analysis. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2023.123927
Wang Jing et al. (2024) Electrospun nanofibrous mats loaded with gemcitabine and cisplatin suppress bladder tumor growth by improving the tumor immune microenvironment. Journal of Materials Science-Materials in Medicine. 10.1007/s10856-024-06786-y
Yanrong Wang et al. (2007) Endocrine function and lymphocyte infiltration of newborn rat ovaries after ultrarapid freezing and allotransplantation. Fertility and Sterility. 10.1016/j.fertnstert.2006.11.063
Xiong Hai-bo et al. (2005) Induction of immune tolerance with heart-thymus composite allotransplantation in rats. Journal of Central South University of Technology. 10.1007/s11771-005-0424-z
