| Field | Specification |
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| Reactivity | |
| Applications | |
| Immunogen | Ni-NTA purified recombinant human CD8 expressed in E. Coli strain BL21 (DE3). |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Storage buffer | |
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Product Overview
This monoclonal antibody, raised in Mouse, recognizes CD8. It is validated for Western Blot, Immunohistochemistry, ELISA.
CD8 T cell surface antigen is heterodimer of an alpha and a beta chain linked by two disulfide bonds .It belongs type I membrane protein. Selectively expressing of CD8 on a subset of T cells leads to CD8 T cell development. Through identifying cytotoxic/ suppressor T-cells that interact with MHC class I bearing targets, CD8 is thought to play a role in the process of T-cell mediated killing. Veillette et al (1988) found the CD8 is associated with the internal membrane tyrosine-protein kinase p56lck.
Background
CD8 T cell surface antigen is heterodimer of an alpha and a beta chain linked by two disulfide bonds .It belongs type I membrane protein. Selectively expressing of CD8 on a subset of T cells leads to CD8 T cell development. Through identifying cytotoxic/ suppressor T-cells that interact with MHC class I bearing targets, CD8 is thought to play a role in the process of T-cell mediated killing. Veillette et al (1988) found the CD8 is associated with the internal membrane tyrosine-protein kinase p56lck.
Validated Applications
| Applications | Western Blot, Immunohistochemistry, ELISA |
|---|
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Ni-NTA purified recombinant human CD8 expressed in E. Coli strain BL21 (DE3).
Specifications
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Reactivity | Human |
| Immunogen | Ni-NTA purified recombinant human CD8 expressed in E. Coli strain BL21 (DE3). |
| Purification | purified by protein A affinity chromatography. |
| Concentration | 1ug/ul |
| Formulation | Liquid |
| Storage Buffer | PBS, pH 7.4 with 0.05% sodium azide. |
| Storage | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Shipping | Ice Pack |
Reactivity Notes
Reactivity: Human. Cross-reactivity with other species has not been fully characterized; validate in your system before critical experiments.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. Contains sodium azide where stated; handle with standard laboratory precautions.
Related Products
Explore related antibodies and detection reagents for CD8 research from Applied Biological Materials (abm) Inc., or contact us for alternative conjugates and formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
