| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-cell surface glycoprotein CD8 alpha chain;T-lymphocyte differentiation antigen T8/Leu-2;CD8a;CD8A;MAL; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD8 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EAF-3 is a rabbit monoclonal antibody that recognizes T-cell surface glycoprotein CD8 alpha chain (gene CD8A) in human and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 25.7 kDa, and the supplier reports an observed band at about 36 kDa.
CD8α (CD8A) is a transmembrane glycoprotein that forms the CD8 co-receptor, usually as a CD8αβ heterodimer on cytotoxic T cells and as a CD8αα homodimer on some other lymphocytes. It binds MHC class I and recruits LCK to enhance T-cell receptor signaling, and it is the standard marker for cytotoxic T cells.
| Target | T-cell surface glycoprotein CD8 alpha chain (gene CD8A; UniProt P01732, human) |
|---|---|
| Clone | EAF-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 25.7 kDa |
| Observed MW | 36 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat kidney tissue, human glioblastoma tissue, human kidney tissue, mouse skin tissue, human colon tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human CD8.
Reactivity Notes
The supplier lists reactivity with human and rat. Tissue expression noted by UniProt (human): CD8 on thymus-derived T-cells usually consists of a disulfide-linked alpha/CD8A and a beta/CD8B chain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiangmei Zhang et al. (2022) Folate Receptor 4-Expressing T cell Is Associated with Disease-Free Survival in Patients with Esophageal Squamous Cell Carcinoma. Disease Markers. 10.1155/2022/4351949
Wanwan Gong et al. (2025) Deciphering ERR family genes as prognostic and immunological biomarkers through pan-cancer analysis with validation in gallbladder cancer. Frontiers in Oncology. 10.3389/fonc.2025.1525635
Jidong Zhao et al. (2021) Tumor Cells Interleukin-22 Expression Associates with Elevated Tumor-Associated Macrophages Infiltrating and Poor Prognosis in Patients with Breast Cancer. Cancer Biotherapy and Radiopharmaceuticals. 10.1089/cbr.2020.3794
Yong Tao et al. (2023) Prognostic impact of CD4+ and CD8+ tumor-infiltrating lymphocytes in patients with colorectal cancer. ACTA Chirurgica Belgica. 10.1080/00015458.2023.2180712
