Anti-Cd80 Antibody Picoband®

Overview

Anti-Cd80 Antibody Picoband® is an antibody for Cd80 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: Cd80 (CD80 molecule); UniProt: Q00609
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 70 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-Cd80 Antibody Picoband® catalog # A00196-3.

Biological background

Biological context: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.

Expression and localization notes: cellular localization: Membrane; Single-pass type I membrane protein., tissue context: Hematopoietic stem and progenitor cell- enriched populations. Found in brain, placenta and testis..

Common research applications

• Western blotting (WB): Compare Cd80 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of Cd80 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify Cd80-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Membrane; Single-pass type I membrane protein.
• Tissue details: Hematopoietic stem and progenitor cell- enriched populations. Found in brain, placenta and testis.
• Research category: Adaptive Immunity,B Cells,Hematopoietic Progenitors,Immunology,Lymphoid,Myeloid,Stem Cells

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.