| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-lymphocyte activation antigen CD80; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7; CD80; CD80; CD28LG; CD28LG1; LAB7 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Cd80 recombinant protein (Position: D62-K92). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes T-lymphocyte activation antigen CD80 in human and mouse samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 34.6 kDa, and the supplier reports an observed band at about 70 kDa.
CD80 (B7-1) is a co-stimulatory ligand on activated antigen-presenting cells. Binding to CD28 on T cells supplies the second signal for full activation, while binding to CTLA-4 delivers inhibitory signals, and it also interacts with PD-L1, placing it at the center of T-cell co-stimulation research.
| Target | T-lymphocyte activation antigen CD80 (gene Cd80; UniProt Q00609, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 34.6 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Daudi cells, human K562 cells, mouse RAW264.7 cells, mouse ANA-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): ANA-1 cells.
Immunogen
Recombinant mouse Cd80 fragment (Asp62–Lys92), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places T-lymphocyte activation antigen CD80 in the cell membrane. Tissue expression noted by UniProt (mouse): Expressed on activated B-cells, gamma interferon stimulated monocytes and non-circulating B-cell malignancies. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ao Hu et al. (2023) Controlled intracellular aggregation of magnetic particles improves permeation and retention for magnetic hyperthermia promotion and immune activation. Theranostics. 10.7150/thno.80821
Gao Liyun et al. (2025) Identification of m6A methyltransferase-related WTAP and ZC3H13 predicts immune infiltrates in glioblastoma. Scientific Reports. 10.1038/s41598-025-88671-4
Ping Chen et al. (2009) Coxsackievirus B3 infection promotes generation of myeloid dendritic cells from bone marrow and accumulation in the myocardium. International Immunopharmacology. 10.1016/j.intimp.2009.07.014
