| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | T-lymphocyte activation antigen CD80;Activation B7-1 antigen;BB1;CTLA-4 counter-receptor B7.1;B7;CD80;CD80;CD28LG, CD28LG1, LAB7; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human CD80 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CD80/B7 1 Rabbit Monoclonal Antibody is an antibody targeting CD80. Common applications include WB, IHC. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AOEH-3; isotype: Rabbit IgG; reactivity: Human; observed MW: 103 kDa; calculated MW: 33048 MW.
Boster Bio Anti-CD80/B7 1 Rabbit Monoclonal Antibody catalog # M00196. Tested in WB, IHC applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CD80 — T-lymphocyte activation antigen CD80
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AOEH-3; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 103 kDa; Calculated: 33048 MW
Biological background
Protein function (datasheet): Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. .
Cellular localization (datasheet): Membrane; Single-pass type I membrane protein.
Tissue details (datasheet): Expressed on activated B-cells, macrophages and dendritic cells.
Research relevance and current trends
- Commonly studied in contexts related to Adaptive Immunity,B Cells,Hematopoietic Progenitors,Immunology,Lymphoid,Myeloid,Stem Cells.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
