| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-lymphocyte activation antigen CD80;Activation B7-1 antigen;BB1;CTLA-4 counter-receptor B7.1;B7;CD80;CD80;CD28LG, CD28LG1, LAB7; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD80 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOEH-3 is a rabbit monoclonal antibody that recognizes T-lymphocyte activation antigen CD80 in human samples and is validated for Western blot and IHC. The predicted molecular weight is 33.0 kDa, and the supplier reports an observed band at about 60 kDa.
CD80 (B7-1) is a co-stimulatory ligand on activated antigen-presenting cells. Binding to CD28 on T cells supplies the second signal for full activation, while binding to CTLA-4 delivers inhibitory signals, and it also interacts with PD-L1, placing it at the center of T-cell co-stimulation research.
| Target | T-lymphocyte activation antigen CD80 (gene CD80; UniProt P33681, human) |
|---|---|
| Clone | AOEH-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 33.0 kDa |
| Observed MW | 60 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human CD80.
Reactivity Notes
The supplier lists reactivity with human. UniProt places T-lymphocyte activation antigen CD80 in the cell membrane. Tissue expression noted by UniProt (human): Expressed on the surfaces of antigen-presenting cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
