| Field | Specification |
|---|---|
| Target | |
| Alternative names | CD83 antigen; hCD83; B-cell activation protein; Cell surface protein HB15; CD antigen CD83; CD83 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD83 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18C52 is a rabbit monoclonal antibody that recognizes CD83 antigen in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 23.0 kDa, and the supplier reports an observed band at about 45 kDa.
CD83 is an immunoglobulin superfamily glycoprotein strongly upregulated on mature dendritic cells and also found on activated B and T cells. It is widely used as a dendritic cell maturation marker, and it helps regulate CD4+ T-cell selection in the thymus and the stability of MHC class II on the cell surface.
| Target | CD83 antigen (gene CD83; UniProt Q01151, human) |
|---|---|
| Clone | 18C52 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 23.0 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Immunogen
Synthetic peptide based on the sequence of human CD83.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places CD83 antigen in the membrane. Tissue expression noted by UniProt (human): Expressed by activated lymphocytes, Langerhans cells and activatd dendritic cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zihong Pan et al. (2019) Subchronic exposure of environmentally relevant concentrations of F-53B in mice resulted in gut barrier dysfunction and colonic inflammation in a sex-independent manner. Environmental Pollution. 10.1016/j.envpol.2019.07.021
Huang Jinyu et al. (2013) S100+ cells: A new neuro-immune cross-talkers in lymph organs. Scientific Reports. 10.1038/srep01114
