| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-lymphocyte activation antigen CD86;Activation B7-2 antigen;B70;BU63;CTLA-4 counter-receptor B7.2;FUN-1;CD86;CD86;CD28LG2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD86 recombinant protein (Position: A24-F329). Human CD86 shares 50% amino acid (aa) sequence identity with mouse CD86. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects T-lymphocyte activation antigen CD86 in human and mouse samples and is validated for IHC and Western blot. The predicted molecular weight is 37.7 kDa, and the supplier reports an observed band at about 60–80 kDa.
CD86 (B7-2) is a co-stimulatory ligand on dendritic cells, macrophages and B cells. It binds CD28 on T cells to provide the second signal for activation and also binds the inhibitory receptor CTLA-4, and its rapid induction makes it a common marker of activated antigen-presenting cells.
| Target | T-lymphocyte activation antigen CD86 (gene CD86; UniProt P42081, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 37.7 kDa |
| Observed MW | 60–80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL |
Samples with a confirmed band (WB): human Raji cells, human Daudi cells, mouse spleen tissue, mouse thymus tissue, mouse J774A1 cells, mouse Ana-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human CD86 fragment (Ala24–Phe329), expressed in E. coli. Sequence identity with the mouse ortholog: 50%.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places T-lymphocyte activation antigen CD86 in the cell membrane. Tissue expression noted by UniProt (human): Expressed on the surfaces of antigen-presenting cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lei Jiahui et al. (2024) AMPK Regulates M1 Macrophage Polarization through the JAK2/STAT3 Signaling Pathway to Attenuate Airway Inflammation in Obesity-Related Asthma. Inflammation. 10.1007/s10753-024-02070-x
Lu-Jin Cheng et al. (2025) Effect of the TIM-3/Gal-9 signaling pathway on macrophage polarization in peri-implantitis. PLoS One. 10.1371/journal.pone.0328258
