| Field | Specification |
|---|---|
| Target | |
| Alternative names | CD9 antigen; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24; CD antigen CD9; CD9; MIC3; TSPAN29 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD9 recombinant protein (Position: Q139-K192). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects CD9 antigen in human samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 25.4 kDa, and the supplier reports an observed band at about 22 kDa.
CD9 is a tetraspanin that associates with integrins and other membrane proteins to influence cell adhesion, migration and platelet activation. It is required for sperm–egg fusion on the oocyte surface, and it is one of the most commonly used exosome markers.
| Target | CD9 antigen (gene CD9; UniProt P21926, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 25.4 kDa |
| Observed MW | 22 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human hcct tissue, human RT4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, human adenocarcinoma of the right colon tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human intestine cancer tissue.
Samples with a confirmed signal (flow cytometry): Jurkat cells.
Immunogen
Recombinant human CD9 fragment (Gln139–Lys192), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes CD9 antigen as secreted and also located in the cell membrane and membrane. Tissue expression noted by UniProt (human): Detected in platelets (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiaohui Chen et al. (2022) Cancer-Derived Small Extracellular Vesicles PICKER. Analytical Chemistry. 10.1021/acs.analchem.2c01683
Liuwan Zhao et al. (2019) Exosome-mediated siRNA delivery to suppress postoperative breast cancer metastasis. Journal of Controlled Release. 10.1016/j.jconrel.2019.12.005
Song Yang et al. (2025) Exosomes derived from human amniotic mesenchymal stem cells promotes angiogenesis in hUVECs by delivering novel miRNA N-194. Molecular Medicine. 10.1186/s10020-025-01192-8
Cong Ma et al. (2025) Mir-574-3p and Mir-125a-5p in Adipose Derived Mesenchymal Stem Cell Exosomes Synergistically Target TGF-β1/Smad2 Signaling Pathway for the Treatment of Androgenic Alopecia. Journal of Investigative Dermatology. 10.1016/j.jid.2025.03.042
Yiping Wang et al. (2025) Evaluation of the Effect of Exosomes From Adipose Derived Stem Cells on Changes in GSH/ROS Levels During Skin Photoaging. Photodermatology Photoimmunology & Photomedicine. 10.1111/phpp.70009
Jiang Lijun et al. (2018) Elevated Serum Exosomal miR-125b Level as a Potential Marker for Poor Prognosis in Intermediate-Risk Acute Myeloid Leukemia. ACTA Haematologica. 10.1159/000491584
