Anti-CD9 Antibody Picoband®

Overview

Anti-CD9 Antibody Picoband® is an antibody for CD9 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: CD9 (microtubule-associated protein 2); UniProt: P21926
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 22 kDa
• Applications: WB, IHC, IF, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-CD9 Antibody Picoband® catalog # A01202-2.

Biological background

Biological context: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

Expression and localization notes: cellular localization: Cytoplasm, cytoskeleton. Cell projection, dendrite..

Common research applications

• Western blotting (WB): Compare CD9 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of CD9 in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify CD9-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: CD9 antigen is a protein that in humans is encoded by the CD9 gene. CD9 is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is found on the surface of exosomes. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance. This protein also seems to be a key part in the egg-sperm fusion during mammalian fertilization. While oocytes are ovulated, CD9-deficient oocytes are not properly fused with sperm upon fertilization. CD9 is located in the microvillar membrane of the oocytes and also appears to intervene in maintaining the normal shape of oocyte microvilli.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cytoplasm, cytoskeleton. Cell projection, dendrite.
• Research category: Cell Type Marker,Cytoskeleton,Cytoskeleton/ECM,Microtubules,Neural Stem Cells,Neuron Marker,Neuroscience,Signal Transduction,Stem Cells

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.