Anti-CD9 Rabbit Monoclonal Antibody

SKU:BHA21007685
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Boster Bio
Boster Bio
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Overview
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Anti-CD9 antibody (Rabbit, Monoclonal, clone Clone: CED-3, Rabbit IgG) recommended for WB, IHC, ICC, IF, IP, Flow Cytometry Commonly used in Immunology & Inflammation studies where these format selectors and application compatibility are required.
Target CD9
Clone number Clone: CED-3
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype Rabbit IgG
Application(s) WB, IHC, ICC, IF, IP, Flow Cytometry
Options selector
Catalog no. Size Conjugation
M01202 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 uL/vial — Unconjugated
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
      Form: Liquid
      Applications: WB,IHC,ICC,IF,IP,Flow Cytometry
      Application details: WB 1:500-2000
      IHC 1:50-200
      ICC/IF 1:50-200
      IP 1:20
      FC 1:20
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No M01202
Alternative Names CD9 antigen;5H9 antigen;Cell growth-inhibiting gene 2 protein;Leukocyte antigen MIC3;Motility-related protein;MRP-1;Tetraspanin-29;Tspan-29;p24;CD9;CD9;MIC3, TSPAN29;GIG2;
Cellular Localization Membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein .
Clonality
  • Monoclonal
Concentration 0.5mg/ml
Host Rabbit
Immunogen A synthesized peptide derived from human CD9
Isotype
  • Rabbit IgG
Molecular Weight 23 kDa
Product Type
  • Antibodies
  • Primary Antibodies
Reactivity
  • Human
  • Mouse
  • Rat
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Target CD9
UniProt # P21926

Overview

Anti-CD9 Rabbit Monoclonal Antibody is an antibody targeting CD9. Common applications include WB, IHC, ICC, IF, IP, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: CED-3; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 23 kDa.

Boster Bio Anti-CD9 Rabbit Monoclonal Antibody catalog # M01202. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

  • Target: CD9 — CD9 antigen
  • Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: CED-3; Isotype: Rabbit IgG
  • Species reactivity: Human,Mouse,Rat
  • Molecular weight guidance: Observed: 23 kDa

Biological background

Protein function (datasheet): Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. .

Cellular localization (datasheet): Membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein .

Tissue details (datasheet): Detected in platelets (at protein level). Expressed by a variety of hematopoietic and epithelial cells. .

Research relevance and current trends

  • Commonly studied in contexts related to Cardiovascular,Cell Type Markers,Developmental Biology,Embryogenesis,Embryonic Stem Cells,Immunology,Intracellular,Platelets,Reproduction,Stem Cells,Surface Molecules.
  • Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
  • Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

  • Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
  • Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
  • Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
  • Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.

Notes for experimental interpretation

  • Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
  • Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

We have seen staining in human leukocyte. What should we do? Is anti-CD9 Rabbit Monoclonal antibody supposed to stain leukocyte positively?
Based on literature leukocyte does express CD9. Based on Uniprot.org, CD9 is expressed in trigeminal ganglion, leukocyte, ovary, platelet, among other tissues. Regarding which tissues have CD9 expression, here are a few articles citing expression in various tissues: Leukocyte, Pubmed ID: 8486348 Ovary, Pubmed ID: 15489334 Platelet, Pubmed ID: 2358073

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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