| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cell division cycle protein 23 homolog;Anaphase-promoting complex subunit 8;APC8;Cyclosome subunit 8;CDC23;ANAPC8; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cdc23/APC8 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IAA-3 is a rabbit monoclonal antibody that recognizes cell division cycle protein 23 homolog (gene CDC23) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 68.8 kDa, and the supplier reports an observed band at about 64 kDa.
CDC23 is a subunit of the anaphase-promoting complex/cyclosome (APC/C), the E3 ubiquitin ligase that degrades securin, mitotic cyclins and other regulators to move cells through mitosis. It belongs to the tetratricopeptide repeat (TPR) arm of the complex and is studied in cell cycle control.
| Target | Cell division cycle protein 23 homolog (gene CDC23; UniProt Q9UJX2, human) |
|---|---|
| Clone | IAA-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 68.8 kDa |
| Observed MW | 64 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HepG2 cells, Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human cdc23/APC8.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
