| Field | Specification |
|---|---|
| Target | |
| Alternative names | M-phase inducer phosphatase 2;3.1.3.48;Dual specificity phosphatase Cdc25B;CDC25B;CDC25HU2; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cdc25B |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HIH-3 is a rabbit monoclonal antibody that recognizes M-phase inducer phosphatase 2 (gene CDC25B) in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 65.0 kDa, and the supplier reports an observed band at about 64 kDa.
CDC25B is a dual-specificity phosphatase that removes inhibitory phosphates from CDK1 and other CDKs to promote entry into mitosis. It acts early in the G2/M transition, is a target of checkpoint kinases after DNA damage, and is frequently overexpressed in tumors.
| Target | M-phase inducer phosphatase 2 (gene CDC25B; UniProt P30305, human) |
|---|---|
| Clone | HIH-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 65.0 kDa |
| Observed MW | 64 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
Immunogen
Synthetic peptide based on the sequence of human cdc25B.
Reactivity Notes
The supplier lists reactivity with human. UniProt places M-phase inducer phosphatase 2 in the cytoplasm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
