| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cell division control protein 42 homolog;G25K GTP-binding protein;CDC42; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CDC42 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IAE-3 is a rabbit monoclonal antibody that recognizes cell division control protein 42 homolog (gene CDC42) in human, mouse and rat samples and is validated for Western blot, IHC, immunoprecipitation and flow cytometry. The predicted molecular weight is 21.3 kDa, and the supplier reports an observed band at about 21 kDa.
CDC42 is a Rho family small GTPase that cycles between active GTP-bound and inactive GDP-bound states. In its active form it engages effectors such as PAK and WASP to control actin organization, filopodia formation, cell polarity and migration, making it a central regulator in cytoskeletal and polarity research.
| Target | Cell division control protein 42 homolog (gene CDC42; UniProt P60953, human) |
|---|---|
| Clone | IAE-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 21.3 kDa |
| Observed MW | 21 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:30 |
| Flow cytometry | 1:50 |
Samples with confirmed staining (IHC): rat skin tissue, human esophageal carcinoma tissue, human colon tissue, human Hodgkin's lymphoma tissue, mouse skeletal muscle - gastrocnemius tissue, mouse skin tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human CDC42.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cell division control protein 42 homolog in the cell membrane, cytoplasm and midbody. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
