| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cell division cycle 5-like protein;Cdc5-like protein;Pombe cdc5-related protein;CDC5L;KIAA0432, PCDC5RP; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CDC5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IOA-3 is a rabbit monoclonal antibody that recognizes cell division cycle 5-like protein (gene CDC5L) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 92.3 kDa, and the supplier reports an observed band at about 95 kDa.
CDC5L is a Myb-related DNA-binding protein that is a core component of the PRP19–CDC5L complex of the spliceosome. It is required for pre-mRNA splicing and also takes part in the DNA damage response, and it has been linked to cell cycle progression at the G2/M transition.
| Target | Cell division cycle 5-like protein (gene CDC5L; UniProt Q99459, human) |
|---|---|
| Clone | IOA-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 92.3 kDa |
| Observed MW | 95 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CDC5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cell division cycle 5-like protein in the nucleus, nucleus speckle and cytoplasm. Tissue expression noted by UniProt (human): Ubiquitously expressed in both fetal and adult tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
