| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cell division control protein 6 homolog;CDC6-related protein;p62 (cdc6);Cdc6; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Recombinant human Cdc6. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CDC6 Antibody (Monoclonal, DCS-180) is an antibody targeting CDC6. Common applications include WB. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: DCS-180; isotype: Mouse IgG1; reactivity: Human; observed MW: 25 kDa; calculated MW: 62614 MW.
Boster Bio Anti-CDC6 Antibody (Monoclonal, DCS-180) catalog # MA1019. Tested in WB applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CDC6 — Cell division control protein 6 homolog
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: DCS-180; Isotype: Mouse IgG1
- Species reactivity: Human
- Molecular weight guidance: Observed: 25 kDa; Calculated: 62614 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Scientific background (datasheet): In yeasts, Cdc6 (Saccharomyces cerevisiae) and Cdc18 (Schizosaccharomyces pombe) associate with the origin recognition complex (ORC) proteins to render cells competent for DNA replication. Cdc6 is overexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication. Yan et al. (1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammalian cells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to a proliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by a functional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2F proteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells.
Cellular localization (datasheet): Nucleus . Cytoplasm . The protein is nuclear in G1 and cytoplasmic in S-phase cells. .
Sequence similarities (datasheet): Belongs to the CDC6/cdc18 family.
Research relevance and current trends
- Commonly studied in contexts related to Cell Biology,Cell Cycle,Cell Division,DNA/RNA,DNA Synthesis,Epigenetics and Nuclear Signaling.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
