| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cell division control protein 6 homolog;CDC6-related protein;Cdc18-related protein;HsCdc18;p62 (cdc6);HsCDC6;CDC6;CDC18L; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cdc6 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HIE-3 is a rabbit monoclonal antibody that recognizes DNA replication factor CDC6 in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 62.7 kDa, and the supplier reports an observed band at about 63 kDa.
CDC6 is an essential DNA replication licensing factor that, together with ORC and CDT1, loads the MCM2–7 helicase onto replication origins in G1. This ensures that each part of the genome is copied once per cell cycle, and CDC6 is studied in replication control and cell proliferation.
| Target | DNA replication factor CDC6 (gene CDC6; UniProt Q99741, human) |
|---|---|
| Clone | HIE-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 62.7 kDa |
| Observed MW | 63 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human cdc6.
Reactivity Notes
The supplier lists reactivity with human. UniProt places DNA replication factor CDC6 in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
