| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cell division cycle 7-related protein kinase;CDC7-related kinase;HsCdc7;huCdc7;2.7.11.1;CDC7;CDC7L1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CDC7 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BGF-3 is a rabbit monoclonal antibody that recognizes cell division cycle 7-related protein kinase (gene CDC7) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 63.9 kDa, and the supplier reports an observed band at about 70 kDa.
CDC7 is a serine/threonine kinase that, together with its regulatory partner DBF4, forms the DDK complex. DDK phosphorylates the MCM2–7 helicase to trigger the firing of replication origins in S phase, making CDC7 essential for DNA replication initiation and a subject of cancer research.
| Target | Cell division cycle 7-related protein kinase (gene CDC7; UniProt O00311, human) |
|---|---|
| Clone | BGF-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 63.9 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CDC7.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cell division cycle 7-related protein kinase in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
