| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Sororin;Cell division cycle-associated protein 5;p35;CDCA5; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human CDCA5 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CDCA5/Sororin Rabbit Monoclonal Antibody is an antibody targeting CDCA5. Common applications include WB, IHC, ICC, IF, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: IAH-3; isotype: Rabbit IgG; reactivity: Human; observed MW: 35 kDa; calculated MW: 27601 MW.
Boster Bio Anti-CDCA5/Sororin Rabbit Monoclonal Antibody catalog # M05043. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CDCA5 — Sororin
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: IAH-3; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 35 kDa; Calculated: 27601 MW
Biological background
Protein function (datasheet): Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair. .
Cellular localization (datasheet): Nucleus . Chromosome . Cytoplasm . Associates with nuclear chromatin from S phase until metaphase and is released in the cytoplasm upon nuclear envelope breakdown.
Tissue details (datasheet): Mainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform 6 are truncated forms expressed in non- neuronal tissues. .
Research relevance and current trends
- Commonly studied in contexts related to Cell Biology,Cell Cycle,Cell Division,Chromosome Structure,Epigenetics and Nuclear Signaling.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
