| Field | Specification |
|---|---|
| Target | |
| Alternative names | CUB domain-containing protein 1;Membrane glycoprotein gp140;Subtractive immunization M plus HEp3-associated 135 kDa protein;SIMA135;Transmembrane and associated with src kinases;CD318;CDCP1;TRASK;UNQ2486/PRO5773; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human CDCP1 recombinant protein (Position: R582-T667). Human CDCP1 shares 84.5% amino acid (aa) sequence identity with mouse CDCP1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes CUB domain-containing protein 1 (gene CDCP1) in human, mouse and rat samples and is validated for flow cytometry, IHC and Western blot. The predicted molecular weight is 92.9 kDa, and the supplier reports an observed band at about 130 kDa.
CDCP1 (CUB domain-containing protein 1) is a transmembrane glycoprotein that is phosphorylated by Src family kinases and signals through PKCδ. It is involved in cell adhesion, anchorage-independent growth and migration, and it is studied as a marker of hematopoietic progenitors and of invasive cancer cells.
| Target | CUB domain-containing protein 1 (gene CDCP1; UniProt Q9H5V8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 92.9 kDa |
| Observed MW | 130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): SW620 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue, rat intestine tissue, human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): PC-3 cells.
Immunogen
Recombinant human CDCP1 fragment (Arg582–Thr667), expressed in E. coli. Sequence identity with the mouse ortholog: 84.5%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Highly expressed in mitotic cells with low expression during interphase. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhu Xiao-Ren et al. (2025) A novel identified epithelial ligand-receptor-associated gene signature highlights POPDC3 as a potential therapy target for non-small cell lung cancer. Cell Death & Disease. 10.1038/s41419-025-07410-9
Badal Chandra Roy et al. (2019) Co-localization of autophagy-related protein p62 with cancer stem cell marker dclk1 may hamper dclk1's elimination during colon cancer development and progression. Oncotarget. 10.18632/oncotarget.26684
