| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CDC28A,CDKN1,P34CDC2,p34 protein kinase,CDK1,Cell division protein kinase 1,Cell division control protein 2 homolog,CDC2,Cyclin-dependent kinase 1 |
| Clonality | |
| Form | Liquid |
| Host | |
| Immunogen | E. coli - derived recombinant Human CDK1 (Met1-Met297). |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Overview
Antibody against CDK1 (Cell division control protein 2 homolog) for research detection of protein expression and localization.
Target biology
CDK1 encodes a cyclin-dependent kinase (CDK) family serine/threonine kinase involved in phosphorylation-driven signaling. CDKs coordinate cell-state transitions by partnering with regulatory proteins and substrates such as RB-family proteins and transcriptional regulators. Antibodies against CDK1 are commonly used to study kinase expression, activation-state changes (often via upstream cues), and pathway rewiring in proliferation and cancer models.
Expected localization
CDK family kinases are typically detected in nuclear and cytoplasmic compartments; distribution can shift with cell-cycle state and binding partners.
Research context
This target is frequently studied in the context of Cell Signaling research. Application suitability (e.g., ELISA, IF, IHC, WB) is indicated in the specifications; conditions should be optimized by titration. Species reactivity information is provided in the specifications (reported: Human, Mouse, Rat).
Recommended experimental notes
- For Western blotting, optimize lysis conditions and include reducing/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.
- For IHC, fixation and antigen retrieval strongly influence epitope accessibility; test citrate- vs EDTA-based retrieval and include matched positive/negative tissues.
- For IF/ICC, adjust permeabilization (e.g., mild detergent vs stronger conditions) based on expected localization; include secondary-only and isotype controls for background.
- For ELISA, ensure capture/detection antibody compatibility and run a standard curve; verify sample matrix effects with spike-and-recovery and dilution linearity.
Controls & interpretation
Where possible, confirm specificity using genetic perturbation (KO/KD/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low/none of CDK1, and a positive control sample known to express CDK1.
Immunogen context
Generated using an immunogen derived from E. coli - derived recombinant Human CDK1 (Met1-Met297).. When interpreting results, note that epitope accessibility can vary with denaturation, fixation, or post‑translational modifications; empirical optimization is recommended.
For research use only. Not for diagnostic procedures.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
