Anti-Cdk2 Antibody Picoband® (monoclonal, 5B12D1)

Overview

Anti-Cdk2 Antibody Picoband® (monoclonal, 5B12D1) is an antibody for CDK2 detection raised in Mouse (Monoclonal, clone Clone: 5B12D1, Mouse IgG2a), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: CDK2 (solute carrier family 2 (facilitated glucose transporter), member 1); UniProt: P24941
• Antibody format: Mouse, Monoclonal, clone Clone: 5B12D1, Mouse IgG2a
• Molecular weight: 30 kDa, calculated 46659 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-Cdk2 Antibody Picoband® (monoclonal, 5B12D1) catalog # M00166-3.

Biological background

Biological context: Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.

Expression and localization notes: cellular localization: Cell membrane. Multi-pass membrane protein. Melanosome., tissue context: Detected in erythrocytes (at protein level). Expressed at variable levels in many human tissues..

Common research applications

• Western blotting (WB): Compare CDK2 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of CDK2 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify CDK2-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: CDK2, Cyclin-Dependent Kinase2, is also known as P33. The CDK2 protein was highly homologous to p34(CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34(cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34(cdc2)/cyclin A. The gene for p33 shares 65% sequence identity with p34(cdc2). P33(cdk2) plays a unique role in cell cycle regulation of vertebrate cells.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane. Multi-pass membrane protein. Melanosome.
• Tissue details: Detected in erythrocytes (at protein level). Expressed at variable levels in many human tissues.
• Research category: Channels,Energy Metabolism,Energy Transfer Pathways,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Plasma Membrane,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.