| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinase; CDK2; CDKN2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Cdk2 recombinant protein (Position: E81-L298). Human Cdk2 shares 98.6% amino acid (aa) sequence identity with rat Cdk2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 5B12D1 is a mouse monoclonal antibody that recognizes cyclin-dependent kinase 2 (gene CDK2) in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 33.9 kDa, and the supplier reports an observed band at about 30 kDa.
CDK2 is a cyclin-dependent kinase that pairs with cyclin E and cyclin A to drive the G1-to-S transition and DNA replication. It phosphorylates targets such as RB1 and helps trigger centrosome duplication; although somatic cells can often compensate for its loss, it is essential for meiosis and is widely studied in cell cycle research.
| Target | Cyclin-dependent kinase 2 (gene CDK2; UniProt P24941, human) |
|---|---|
| Clone | 5B12D1 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2a |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 33.9 kDa |
| Observed MW | 30 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
Samples with a confirmed band (WB): human Jurkat cells, human HepG2 cells, human K562 cells, rat heart tissue, rat L6 cells, mouse heart tissue, mouse C2C12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human laryngeal squamous cells carcinomas tissue, human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): ANA-1 cells.
Immunogen
Recombinant human Cdk2 fragment (Glu81–Leu298), expressed in E. coli. Sequence identity with the rat ortholog: 98.6%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cyclin-dependent kinase 2 in the nucleus, cytoplasm and endosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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