| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3; CDK4 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant human Cdk4 protein. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DCS-31 is a mouse monoclonal antibody that recognizes cyclin-dependent kinase 4 (gene CDK4) in human, mouse and rat samples and is validated for Western blot and ICC/IF.
CDK4 is a serine/threonine kinase that partners with D-type cyclins to phosphorylate the retinoblastoma protein RB1 in G1 phase. This releases E2F transcription factors and commits the cell to enter S phase, which is why CDK4 and its inhibitors are central topics in cell cycle and cancer research.
| Target | Cyclin-dependent kinase 4 (gene CDK4; UniProt P11802, human) |
|---|---|
| Clone | DCS-31 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2a |
| Reactivity | Human, Mouse, Rat |
| Purification | Ascites |
| Form | Lyophilized |
| Formulation | 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers |
| Reconstitution | 100 µg/mL after adding 1 mL PBS |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | Not specified by the supplier |
Immunogen
Recombinant human Cdk4 protein.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cyclin-dependent kinase 4 in the cytoplasm, nucleus and nuclear membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yanshan Ge et al. (2018) CD38 affects the biological behavior and energy metabolism of nasopharyngeal carcinoma cells. International Journal of Oncology. 10.3892/ijo.2018.4651
