Anti-Cdk6 Antibody Picoband®

SKU:BHA21000900
Research Validated
—
Suppliers
Boster Bio
Boster Bio
Details Products
Overview
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Anti-CDK6 antibody (Rabbit polyclonal, Rabbit IgG). Validated for immunohistochemistry and Western blot. Reactivity: Human, Rat (species coverage varies by application). Recognizes cyclin-dependent kinase 6. Observed band in Western blot: about 39 kDa.
Target CDK6
Host Rabbit
Reactivity Human,Rat
Isotype Rabbit IgG
Application(s) IHC, WB
Options selector
Catalog no. Size Conjugation
PB9995 100 ug/vial
Available Options

Select the variant that best fits your experiment.

  • Options:
    • 100 ug/vial / Carrier Free, Unconjugated
      Form: Lyophilized
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
      Applications: IHC,WB
      Application details: Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
      Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
    • 100 ug/vial / APC, Biotin, Cy3, FITC, Fluoro488, Fluoro550, Fluoro594, Fluoro647, PE
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.; At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Applications: WB,IHC,ELISA; Flow Cytometry
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cellsContents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 100 ug/vial / HRP
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: varies by selected option; see option details under Options.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target CDK6
Alternative names Cyclin-dependent kinase 6;2.7.11.22;Cell division protein kinase 6;Serine/threonine-protein kinase PLSTIRE;CDK6;CDKN6;
UniProt # Q00534
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
  • Rat
Applications
  • Immunohistochemistry
  • Western Blot
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human Cdk6, identical to the related mouse sequence.
Molecular weight 36.9 kDa
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Cytoplasm. Nucleus. Cell projection, ruffle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Localized to the ruffling edge of spreading fibroblasts. Kinase activity only in nucleus. Localized to the cytosol of neurons and showed prominent staining around either side of the nucleus (By similarity). Present in the cytosol and in the nucleus in interphase cells and at the centrosome during mitosis from prophase to telophase (PubMed:23918663). .
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) PB9995
Main SKU BHA21000900
Rabbit Polyclonal · Picoband®

Product Overview

This rabbit polyclonal antibody recognizes cyclin-dependent kinase 6 (gene CDK6) in human and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 36.9 kDa, and the supplier reports an observed band at about 39 kDa.

CDK6 is a serine/threonine kinase that forms complexes with D-type cyclins to phosphorylate RB1 and move cells from G1 into S phase. Besides its cell cycle role it influences differentiation, particularly in blood cell development, and it is a key target in cancer research alongside CDK4.

Target Cyclin-dependent kinase 6 (gene CDK6; UniProt Q00534, human)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Human, Rat
Form Lyophilized
Formulation Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers
Calculated MW 36.9 kDa
Observed MW 39 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 0.1–0.5 µg/mL (Human, Rat)
IHC (paraffin sections) 0.5–1 µg/mL (Human)

Samples with confirmed staining (IHC): human intestinal cancer tissue, human mammary cancer tissue, human tonsil tissue.

Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.

Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).

Immunogen

Synthetic peptide from the N-terminal region of human cdk6. The peptide sequence is conserved in mouse.

Reactivity Notes

The supplier lists reactivity with human and rat. UniProt places cyclin-dependent kinase 6 in the cytoplasm, nucleus and cell projection. Tissue expression noted by UniProt (human): Expressed ubiquitously. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 0.1–0.5 µg/mL (Human, Rat); IHC (paraffin sections) 0.5–1 µg/mL (Human). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Rat. Other species are not listed by the supplier.
Q.Is a blocking reagent available for this antibody?
A.A matching blocking reagent for competition (specificity) controls can be supplied on request. Contact info@biohippo.com to request it.
Q.Is a carrier-free (BSA-free) version available, for example for conjugation?
A.Yes. A carrier-free formulation without BSA, stabilized with trehalose and/or glycerol, can be prepared on request and is compatible with common conjugation chemistries. Please note this when ordering or contact info@biohippo.com. For storage at −20 °C, keep a cryoprotectant such as glycerol in the buffer rather than PBS alone.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 36.9 kDa; observed band at about 39 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide from the N-terminal region of human cdk6. The peptide sequence is conserved in mouse.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Lu Zongliang et al. (2021) Combined Anti-Cancer Effects of Platycodin D and Sorafenib on Androgen-Independent and PTEN-Deficient Prostate Cancer. Frontiers in Oncology. 10.3389/fonc.2021.648985

Donglei Sun et al. (2021) Triclosan down-regulates fatty acid synthase through microRNAs in HepG2 cells. European Journal of Pharmacology. 10.1016/j.ejphar.2021.174261

Chuanchang Li et al. (2017) Effects of miR-1236-3p and miR-370-5p on activation of p21 in various tumors and its inhibition on the growth of lung cancer cells. Tumor Biology. 10.1177/1010428317710824

Yueling Tang et al. (2023) Role and mechanism of Circ-PDE7B in the formation of keloid. International Wound Journal. 10.1111/iwj.14269

Cai Ceng et al. (2025) Association Between CDK4/CDK6 Expression and Prognosis in Patients with Adenocarcinoma of Gastroesophageal Junction. Digestive Diseases and Sciences. 10.1007/s10620-025-09136-2

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