Anti-CDKN2A/p14ARF Rabbit Monoclonal Antibody

SKU:BHA21009425
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-CDKN2A antibody (Rabbit monoclonal, clone 19C26, IgG). Validated for Western blot, immunofluorescence, immunoprecipitation and flow cytometry. Reactivity: Human. Observed band in Western blot: about 14 kDa.
Target CDKN2A
clone number 19C26
Host Rabbit
Reactivity Human
Isotype IgG
Application(s) WB, ICC, IF, IP, Flow
Options selector
Catalog no. Size Conjugation
M00016-2 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,ICC,IF,IP,Flow Cytometry
      Application details: WB 1:500-2000<br>ICC/IF 1:50-200<br>IP 1:50<br>FC 1:80<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target CDKN2A
Alternative names Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF;
Gene ID 1029
UniProt # P42771/Q8N726
Host Rabbit
Clone 19C26
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
Applications
  • Western Blot
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
Immunogen A synthesized peptide derived from human CDKN2A/p14ARF
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M00016-2
Main SKU BHA21009425
Rabbit Monoclonal

Product Overview

Clone 19C26 is a rabbit monoclonal antibody that recognizes CDKN2A in human samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry.

Target CDKN2A (UniProt P42771/Q8N726)
Clone 19C26
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Observed MW 14 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunocytochemistry / Immunofluorescence 1:50–1:200
Immunoprecipitation 1:50
Flow cytometry 1:80

Immunogen

Synthetic peptide based on the sequence of human CDKN2A/p14ARF.

Reactivity Notes

The supplier lists reactivity with human.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunocytochemistry / Immunofluorescence 1:50–1:200; Flow cytometry 1:80; Immunoprecipitation 1:50. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: observed band at about 14 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human CDKN2A/p14ARF.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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