| Field | Specification |
|---|---|
| Target | |
| Alternative names | Carf; Als2cr8; Calcium-responsive transcription factor; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8 protein; Calcium-response factor; CaRF |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse CDKN2AIP/CARF recombinant protein (Position: R183-T689). Human CDKN2AIP/CARF shares 78.3% and 88.1% amino acid (aa) sequence identity with human and rat CDKN2AIP/CARF, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects calcium-responsive transcription factor (gene Carf) in mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
In mouse, CaRF (calcium-responsive transcription factor) is a neuron-enriched transcription factor that binds the calcium-response element CaRE1 in the Bdnf promoter. It helps drive calcium- and activity-dependent BDNF transcription, and it is studied in neuronal activity-regulated gene expression.
| Target | Calcium-responsive transcription factor (gene Carf; UniProt Q8VHI4, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant mouse CDKN2AIP/CARF fragment (Arg183–Thr689), expressed in E. coli. Sequence identity with the human and rat orthologs: 78.3% and 88.1%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places calcium-responsive transcription factor in the nucleus. Tissue expression noted by UniProt (mouse): Highly expressed in brain and testis.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
