| Field | Specification |
|---|---|
| Target | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A BALB/c mouse was immunized with human B-cell line Daudi.Fusion partner: P3-X63-Ag8.653. |
| Conjugate | |
| Purity | |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This monoclonal antibody (clone IPO-10), raised in Mouse, recognizes CDw78/MHC II DR. It is validated for ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry.
Validated Applications
| Applications | ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry |
|---|---|
| ELISA (solid phase | 0, 1-100 µg/ml; tracer: 0, 001-100 µg/ml for 30 min at RT). Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
A BALB/c mouse was immunized with human B-cell line Daudi.Fusion partner: P3-X63-Ag8.653.
Specifications
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone | IPO-10 |
| Isotype | IgG3k |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Immunogen | A BALB/c mouse was immunized with human B-cell line Daudi.Fusion partner: P3-X63-Ag8.653. |
| Purification | Affinity purified from tissue culture. |
| Purity | The antibody is purified from tissue culture and contains no host Ig. |
| Concentration | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Formulation | PBS with 0.02% Sodium Azide. |
| Storage | Store for up to 1 year at 2-8°C upon receipt. |
Reactivity Notes
Validated reactivity: Human. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for CDw78/MHC II DR research from St John's Laboratory Ltd, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
