| Field | Specification |
|---|---|
| Target | |
| Alternative names | Carcinoembryonic antigen-related cell adhesion molecule 5;Carcinoembryonic antigen;CEA;Meconium antigen 100;CD66e;CEACAM5;CEA; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CEA (CD66e) |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAOA-3 is a rabbit monoclonal antibody that recognizes cell adhesion molecule CEACAM5 in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 76.8 kDa, and the supplier reports an observed band at about 180–220 kDa.
CEACAM5, better known as carcinoembryonic antigen (CEA, CD66e), is a GPI-anchored glycoprotein of the immunoglobulin superfamily. It mediates cell adhesion with itself and related CEACAMs, and because it is often highly expressed in colorectal and other carcinomas, it is one of the most widely studied tumor-associated antigens.
| Target | Cell adhesion molecule CEACAM5 (gene CEACAM5; UniProt P06731, human) |
|---|---|
| Clone | AAOA-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 76.8 kDa |
| Observed MW | 180–220 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human colon cancer.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human colon carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human CEA (CD66e).
Reactivity Notes
The supplier lists reactivity with human. UniProt places cell adhesion molecule CEACAM5 in the cell membrane, apical cell membrane and cell surface. Tissue expression noted by UniProt (human): Expressed in columnar epithelial and goblet cells of the colon (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jianwen Sheng et al. (2025) A dynamic molecular landscape in colorectal cancer progression at single-cell resolution. Journal of Translational Medicine. 10.1186/s12967-025-06785-9
