Anti-Ceacam1 Antibody Picoband®

SKU:BHA21006335
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Suppliers
Boster Bio
Boster Bio
Details Products
Overview
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Anti-Ceacam1 antibody (Rabbit polyclonal, IgG). Validated for Western blot, immunofluorescence, flow cytometry and ELISA. Reactivity: Mouse, Rat (species coverage varies by application). Recognizes cell adhesion molecule CEACAM1.
Target Ceacam1
Host Rabbit
Reactivity Mouse,Rat
Isotype IgG
Application(s) WB, IHC, ICC, IF, Flow Cytometry, ELISA
Options selector
Catalog no. Size Conjugation
A00923-3 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 ug/vial — Unconjugated; 100 ug/vial — Carrier Free
      Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
      Form: Lyophilized
      Applications: WB,ICC,IF,Flow Cytometry,ELISA
      Application details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
      Immunocytochemistry/Immunofluorescence, 5 μg/ml, Mouse
      Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
      ELISA, 0.1-0.5 μg/ml, -
      Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
    • 100 ug/vial — Biotin; 100 ug/vial — Cy3; 100 ug/vial — Fluoro488; 100 ug/vial — Fluoro550; 100 ug/vial — Fluoro594; 100 ug/vial — FITC; 100 ug/vial — APC; 100 ug/vial — PE; 100 ug/vial — Fluoro647
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
      Form: Liquid
      Applications: Flow Cytometry | WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cells
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
    • 100 ug/vial — HRP
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
      Form: Liquid
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Ceacam1
Alternative names Cell adhesion molecule CEACAM1; Biliary glycoprotein 1; BGP-1; Biliary glycoprotein D; Carcinoembryonic antigen-related cell adhesion molecule 1; CEA cell adhesion molecule 1; MHVR1; Murine hepatitis virus receptor; MHV-R; CD antigen CD66a; Ceacam1; Bgp; Bgp1
UniProt # P31809
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunofluorescence
  • Flow Cytometry
  • ELISA
Immunogen E.coli-derived mouse Ceacam1 recombinant protein (Position: A34-D382). Mouse Ceacam1 shares 55.5% and 68.8% amino acid (aa) sequence identity with human and rat Ceacam1, respectively.
Purification Immunogen affinity purified.
Reconstitution Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Cellular localization [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Lateral cell membrane. Apical cell membrane. Basal cell membrane. Cell junction. Cell junction, adherens junction.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Catalog no. (Mfr.) A00923-3
Main SKU BHA21006335
Rabbit Polyclonal · Picoband®

Product Overview

This rabbit polyclonal antibody targets cell adhesion molecule CEACAM1 in mouse and rat samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA.

CEACAM1 (CD66a) is an immunoglobulin-superfamily adhesion molecule of the carcinoembryonic antigen family, found on epithelial, endothelial and immune cells. Besides mediating cell-cell adhesion, it acts as an inhibitory receptor in T cells and other immune cells and influences insulin clearance and angiogenesis, making it a subject of immunology and cancer research.

Target Cell adhesion molecule CEACAM1 (gene Ceacam1; UniProt P31809, mouse)
Host / Clonality / Isotype Rabbit / Polyclonal / IgG
Reactivity Mouse, Rat
Purification Immunogen affinity purified
Form Lyophilized
Formulation Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4
Reconstitution 500 µg/mL after adding 0.2 mL distilled water
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 0.25–0.5 µg/mL (Mouse, Rat)
Immunocytochemistry / Immunofluorescence 5 µg/mL (Mouse)
Flow cytometry (fixed cells) 1–3 µg/1×106 cells (Mouse)
ELISA 0.1–0.5 µg/mL

Immunogen

Recombinant mouse Ceacam1 fragment (Ala34–Asp382), expressed in E. coli. Sequence identity with the human and rat orthologs: 55.5% and 68.8%, respectively.

Reactivity Notes

The supplier lists reactivity with mouse and rat. Tissue expression noted by UniProt (mouse): Expressed in granulocytes, lymphocytes, granulocytes, B cells, and T-cells.

Safety

For research use only; not for diagnostic or therapeutic procedures. Handle with standard laboratory precautions.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 0.25–0.5 µg/mL (Mouse, Rat); Immunocytochemistry / Immunofluorescence 5 µg/mL (Mouse); Flow cytometry (fixed cells) 1–3 µg/1×10⁶ cells (Mouse); ELISA 0.1–0.5 µg/mL. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Mouse, Rat. Other species are not listed by the supplier.
Q.What immunogen was used to raise this antibody?
A.Recombinant mouse Ceacam1 fragment (Ala34–Asp382), expressed in E. coli. Sequence identity with the human and rat orthologs: 55.5% and 68.8%, respectively.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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