| Field | Specification |
|---|---|
| Target | |
| Alternative names | CCAAT/enhancer-binding protein alpha ;C/EBP alpha ;CEBPA ;CEBP ; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CEBP alpha |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FIE-3 is a rabbit monoclonal antibody that recognizes CCAAT/enhancer-binding protein alpha (gene CEBPA) in human, mouse and rat samples and is validated for Western blot, immunoprecipitation and flow cytometry. The predicted molecular weight is 37.6 kDa, and the supplier reports an observed band at about 43 kDa.
C/EBPα (CEBPA) is a basic leucine zipper transcription factor that couples growth arrest with terminal differentiation. It drives the development of granulocytes, adipocytes and hepatocytes and regulates metabolic genes in the liver, and it is extensively studied in myeloid differentiation and acute myeloid leukemia.
| Target | CCAAT/enhancer-binding protein alpha (gene CEBPA; UniProt P49715, human) |
|---|---|
| Clone | FIE-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 37.6 kDa |
| Observed MW | 43 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:1000 |
|---|---|
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): U937 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CEBP alpha.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places CCAAT/enhancer-binding protein alpha in the nucleus and SUBCELLULAR LOCATION:. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
