| Field | Specification |
|---|---|
| Target | |
| Alternative names | CCAAT/enhancer-binding protein beta ;C/EBP beta ;Liver activator protein;LAP;Liver-enriched inhibitory protein;LIP;Nuclear factor NF-IL6;Transcription factor 5;TCF-5;CEBPB ;TCF5;PP9092; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CEBP Beta |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAG-3 is a rabbit monoclonal antibody that recognizes CCAAT/enhancer-binding protein beta (gene CEBPB) in human, mouse and rat samples and is validated for Western blot, immunoprecipitation and flow cytometry. The predicted molecular weight is 36.1 kDa, and the supplier reports an observed band at about 33 kDa.
C/EBPβ (CEBPB) is a basic leucine zipper transcription factor that controls many immune and inflammatory genes, including those induced by IL-6 and IL-1. It also regulates adipogenesis, liver metabolism and regeneration, and its isoforms made from alternative start sites (LAP and LIP) have opposing activities.
| Target | CCAAT/enhancer-binding protein beta (gene CEBPB; UniProt P17676, human) |
|---|---|
| Clone | AAG-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 36.1 kDa |
| Observed MW | 33 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): PC-12 cells, human spleen.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CEBP Beta.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places CCAAT/enhancer-binding protein beta in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed at low levels in the lung, kidney and spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wei Wei et al. (2023) METTL16 promotes glycolytic metabolism reprogramming and colorectal cancer progression. Journal of Experimental & Clinical Cancer Research. 10.1186/s13046-023-02732-y
