Anti-Cenexin1 / ODF2 Rabbit Monoclonal Antibody

SKU:BHA21009607
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-ODF2 antibody (Rabbit monoclonal, clone 21O10, IgG). Validated for Western blot and flow cytometry. Reactivity: Human. Recognizes outer dense fiber protein 2. Observed band in Western blot: about 95 kDa.
Target ODF2
clone number 21O10
Host Rabbit
Reactivity Human
Isotype IgG
Application(s) WB, Flow
Options selector
Catalog no. Size Conjugation
M05599 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,Flow Cytometry
      Application details: WB 1:500-2000<br>FC 1:100<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target ODF2
Alternative names Outer dense fiber protein 2; Cenexin; Outer dense fiber of sperm tails protein 2; ODF2
UniProt # Q5BJF6
Host Rabbit
Clone 21O10
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
Applications
  • Western Blot
  • Flow Cytometry
Immunogen A synthesized peptide derived from human Cenexin1 / ODF2
Molecular weight 95.4 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Cytoplasm . Cytoplasm, cell cortex . Cell junction . Cell projection . Cell projection, growth cone . In the absence of antigen, evenly distributed throughout subcortical regions of the T-cell membrane and cytoplasm. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation clusters (SMAC) (PubMed:20215400). Colocalized with DBN1, RUFY3 and F-actin at the transitional domain of the axonal growth cone (By similarity). .
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M05599
Main SKU BHA21009607
Rabbit Monoclonal

Product Overview

Clone 21O10 is a rabbit monoclonal antibody that recognizes outer dense fiber protein 2 (gene ODF2) in human samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 95.4 kDa, and the supplier reports an observed band at about 95 kDa.

ODF2 (cenexin) is a component of sperm tail outer dense fibers and also a scaffold protein at the distal and subdistal appendages of the mother centriole. At the centriole it is needed to anchor microtubules and to build primary cilia, so it is studied in both sperm biology and ciliogenesis.

Target Outer dense fiber protein 2 (gene ODF2; UniProt Q5BJF6, human)
Clone 21O10
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 95.4 kDa
Observed MW 95 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Flow cytometry 1:100

Immunogen

Synthetic peptide based on the sequence of human cenexin1 / ODF2.

Reactivity Notes

The supplier lists reactivity with human. UniProt places outer dense fiber protein 2 in the cell projection and cytoplasm. Tissue expression noted by UniProt (human): Testis-specific (at protein level).

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Flow cytometry 1:100. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 95.4 kDa; observed band at about 95 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human cenexin1 / ODF2.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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