| Field | Specification |
|---|---|
| Target | |
| Alternative names | CEP135; CEP4; KIAA0635; Centrosomal protein of 135 kDa; Cep135; Centrosomal protein 4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CEP135 recombinant protein (Position: E28-E1034). Human CEP135 shares 82.8% amino acid (aa) sequence identity with mouse CEP135. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes centrosomal protein of 135 kDa (gene CEP135) in human, mouse and rat samples and is validated for Western blot and ELISA.
CEP135 is a centrosomal microtubule-binding protein that acts as a scaffold during early centriole assembly. It links central centriole components to microtubules and supports centriole duplication and centrosome integrity; mutations in CEP135 are associated with primary microcephaly.
| Target | Centrosomal protein of 135 kDa (gene CEP135; UniProt Q66GS9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human CEP135 fragment (Glu28–Glu1034), expressed in E. coli. Sequence identity with the mouse ortholog: 82.8%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
