Anti-CEP43 Rabbit Monoclonal Antibody

SKU:BHA21010429
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Overview
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Anti-FGFR1OP antibody (Rabbit monoclonal, clone 29F39, IgG). Validated for Western blot and immunofluorescence. Reactivity: Human, Mouse, Rat. Recognizes centrosomal protein 43. Observed band in Western blot: about 50 kDa.
Target FGFR1OP
clone number 29F39
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype IgG
Application(s) WB, IF, ICC
Options selector
Catalog no. Size Conjugation
M06907 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      • Form: Liquid
      • Applications: WB,ICC,IF
      • Application details: WB 1:500-2000
        ICC/IF 1:50-200
      • Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: Refer to the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: follow the product datasheet storage instructions.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target FGFR1OP
Alternative names MAD2L1-binding protein;Caught by MAD2 protein;MAD2L1BP;CMT2, KIAA0110;
Gene ID 11116
UniProt # O95684
Host Rabbit
Clone 29F39
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunofluorescence
Immunogen A synthesized peptide derived from human CEP43
Molecular weight 43.1 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Nucleus. Cytoplasm, cytoskeleton, spindle. During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M06907
Main SKU BHA21010429
Rabbit Monoclonal

Product Overview

Clone 29F39 is a rabbit monoclonal antibody that recognizes centrosomal protein 43 (gene CEP43) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 43.1 kDa, and the supplier reports an observed band at about 50 kDa.

CEP43 (also known as FOP or FGFR1OP) is a centrosomal protein needed to anchor microtubules at the centrosome. It is also required for primary cilium formation, and it is studied in centrosome biology and ciliogenesis.

Target Centrosomal protein 43 (gene CEP43; UniProt O95684, human)
Clone 29F39
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 43.1 kDa
Observed MW 50 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunocytochemistry / Immunofluorescence 1:50–1:200

Samples with a confirmed band (WB): HeLa cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human CEP43.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunocytochemistry / Immunofluorescence 1:50–1:200. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 43.1 kDa; observed band at about 50 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human CEP43.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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