| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cholesteryl ester transfer protein; Lipid transfer protein I; CETP |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CETP recombinant protein (Position: H77-K353). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CETP Antibody Picoband® is an antibody for CETP detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: CETP (cholesteryl ester transfer protein); UniProt: P11597
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 75 kDa, calculated 63525 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-CETP Antibody Picoband® catalog # A00309-1.
Biological background
Biological context: Involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles. Allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL (PubMed:3600759, PubMed:24293641). Regulates the reverse cholesterol transport, by which excess cholesterol is removed from peripheral tissues and returned to the liver for elimination (PubMed:17237796).
Expression and localization notes: cellular localization: Isoform 1: Cell membrane ; Single- pass type I membrane protein . Late endosome . Lysosome . Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes. ., tissue context: Expressed by the liver and secreted in plasma..
Common research applications
- Western blotting (WB): Compare CETP levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of CETP in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify CETP-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: CETP (Cholesteryl Ester Transfer Protein Plasma), is a plasma protein that facilitates the transport of cholesteryl esters and triglycerides between the lipoproteins. CETP is also known as lipid transfer protein I (Day et al., 1994). Sparkes et al. (1987) used a CETP probe against DNA from a human/mouse somatic cell hybrid panel to assign the CETP gene to chromosome 16. Because the role of CETP in atherosclerosis remained unclear, Okamoto et al. (2000) attempted to develop a potent, specific CETP inhibitor. One inhibitor, JTT-705, forms a disulfide bond with CETP and increases high density lipoprotein (HDL) cholesterol, decreases non-HDL cholesterol, and inhibits the progression of atherosclerosis in rabbits.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Isoform 1: Cell membrane ; Single- pass type I membrane protein . Late endosome . Lysosome . Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes. .
- Tissue details: Expressed by the liver and secreted in plasma.
- Research category: Cancer,Cytokines,Epigenetics and Nuclear Signaling,Immunology,Innate Immunity,Interferons,Nuclear Signaling,Nuclear Signaling Pathways,Signal Transduction,Signaling Pathway,Tumor Immunology
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
