| Field | Specification |
|---|---|
| Target | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | GFP from the jellyfish Aequorea victoria N-Terminal peptide-KLH conjugates (first 20 aa) |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Storage buffer | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This monoclonal antibody (clone 1218), raised in Mouse, recognizes CFP Tag. Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP. It is validated for Western Blot, ELISA, Immunoprecipitation.
Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have fun
Background
Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
Validated Applications
| Applications | Western Blot, ELISA, Immunoprecipitation |
|---|
WB 1:1000. For other applications, the researcher should determine the optimal working dilution.
Immunogen
GFP from the jellyfish Aequorea victoria N-Terminal peptide-KLH conjugates (first 20 aa)
Specifications
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone | 1218 |
| Isotype | IgG |
| Reactivity | Other |
| Immunogen | GFP from the jellyfish Aequorea victoria N-Terminal peptide-KLH conjugates (first 20 aa) |
| Purification | Affinity purified |
| Specificity | Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP. |
| Concentration | 100ug/100ul |
| Formulation | Liquid |
| Storage Buffer | PBS, pH 7.4 with 0.02% sodium azide. |
| Storage | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Shipping | Ice Pack |
Reactivity Notes
Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. Contains sodium azide where stated; handle with standard laboratory precautions.
Related Products
Explore related antibodies and detection reagents for CFP Tag research from Applied Biological Materials (abm) Inc., or contact us for alternative conjugates and formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
