| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclic GMP-AMP synthase; cGAMP synthase; cGAS; h-cGAS; 2'3'-cGAMP synthase; Mab-21 domain-containing protein 1; C6orf150; MB21D1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CGAS recombinant protein (Position: L208-F522). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects cyclic GMP-AMP synthase (gene CGAS) in human samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 58.8 kDa, and the supplier reports an observed band at about 62 kDa.
cGAS (cyclic GMP-AMP synthase) is a cytosolic DNA sensor of the innate immune system. When it binds double-stranded DNA from pathogens or damaged cells, it produces the second messenger 2′3′-cGAMP, which activates STING and drives type I interferon and inflammatory responses.
| Target | Cyclic GMP-AMP synthase (gene CGAS; UniProt Q8N884, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 58.8 kDa |
| Observed MW | 62 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Caco-2 cells, human A431 cells, human HeLa- WT cells, human Hela-CGAS KO cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): Caco-2 cells.
Samples with a confirmed signal (flow cytometry): THP-1 cells, U937 cells.
Immunogen
Recombinant human CGAS fragment (Leu208–Phe522), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places cyclic GMP-AMP synthase in the nucleus, chromosome, cell membrane and cytoplasm. Tissue expression noted by UniProt (human): Expressed in the monocytic cell line THP1. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Gong Zhaoyang et al. (2025) Hydrogel loaded with cerium-manganese nanoparticles and nerve growth factor enhances spinal cord injury repair by modulating immune microenvironment and promoting neuronal regeneration. Journal of Nanobiotechnology. 10.1186/s12951-025-03098-3
Qu Yali et al. (2025) cGAS mRNA-based Immune Agonist Promotes Vaccine Responses and Antitumor Immunity. Cancer Immunology Research. 10.1158/2326-6066.CIR-24-0804
Zhou Shengyang et al. (2024) The cGAS-STING-interferon regulatory factor 7 pathway regulates neuroinflammation in Parkinson’s disease. Neural Regeneration Research. 10.4103/NRR.NRR-D-23-01684
Wenyuan Li et al. (2024) Inhibition of inflammation and apoptosis through the cyclic GMP-AMP synthase-stimulator of interferon genes pathway by stress granules after ALKBH5 demethylase activation during diabetic myocardial ischaemia-reperfusion injury. Diabetes Obesity & Metabolism. 10.1111/dom.15743
Ming-Wei Fan et al. (2026) Electroacupuncture protects gastric Cajal cells by reducing macrophage pyroptosis in diabetic gastroparesis. World Journal of Diabetes. 10.4239/wjd.v17.i2.114252
Xiang Cai et al. (2024) JMJD2A promotes the development of castration-resistant prostate cancer by activating androgen receptor enhancer and inhibiting the cGAS-STING pathway. Molecular Carcinogenesis. 10.1002/mc.23753
