| Field | Specification |
|---|---|
| Target | |
| Alternative names | cGMP-dependent protein kinase 1; cGK 1; cGK1; cGMP-dependent protein kinase I; cGKI; PRKG1; PRKG1B; PRKGR1A; PRKGR1B |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human cGKI/PRKG1 recombinant protein (Position: S2-Q44). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes cGMP-dependent protein kinase 1 (gene PRKG1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 76.4 kDa, and the supplier reports an observed band at about 78 kDa.
PKG1 (PRKG1, also called cGKI) is the main effector kinase of nitric oxide/cGMP signaling. When cGMP binds, it phosphorylates proteins that lower intracellular Ca2+ and reduce calcium sensitivity of the contractile machinery, promoting smooth muscle relaxation and inhibiting platelet activation, so it is central to vascular research.
| Target | CGMP-dependent protein kinase 1 (gene PRKG1; UniProt Q13976, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 76.4 kDa |
| Observed MW | 78 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat lung tissue, rat heart tissue, mouse lung tissue, human HEK293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse lung tissue, rat lung tissue, hman breast cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): RH35 cells, U251 cells.
Immunogen
Recombinant human cGKI/PRKG1 fragment (Ser2–Gln44), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cGMP-dependent protein kinase 1 in the cytoplasm. Tissue expression noted by UniProt (human): Primarily expressed in lung and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zeyu Zhang et al. (2026) Optimized New Shengmai Powder suppresses ferroptosis in ischemic cardiomyocytes via cGMP-PKG signalling. Chinese Medicine. 10.1186/s13020-026-01364-6
Jiahui Wang et al. (2023) Diet Supplementation of Luteolin before Fatty Liver Formation Improves Hepatic Steatosis in Obese Mice by Inhibiting Visceral Adipose Tissue Lipolysis. Molecular Nutrition & Food Research. 10.1002/mnfr.202200478
Yang Zhan Ting et al. (2026) Modulation of vascular remodeling and vasoconstriction by α-phellandrene in hypoxia-induced pulmonary hypertension. Journal of Pharmacy and Pharmacology. 10.1093/jpp/rgag046
