| Field | Specification |
|---|---|
| Target | |
| Alternative names | Choline O-acetyltransferase;CHOACTase;ChAT;Choline acetylase;2.3.1.6;CHAT; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CHAT |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABBO-3 is a rabbit monoclonal antibody that recognizes choline O-acetyltransferase (gene CHAT) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 82.5 kDa; the supplier reports observed bands at 70, 82 kDa.
Choline acetyltransferase (ChAT) synthesizes the neurotransmitter acetylcholine from choline and acetyl-CoA. It is the defining marker of cholinergic neurons, including motor neurons and basal forebrain neurons, and is widely used in neuroscience research.
| Target | Choline O-acetyltransferase (gene CHAT; UniProt P28329, human) |
|---|---|
| Clone | ABBO-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 82.5 kDa |
| Observed MW | 70, 82 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human CHAT.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jin-hong Wang et al. (2014) Catalpol regulates cholinergic nerve system function through effect on choline acetyl-transferase not M receptor affinity. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2014.12.014
