| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP-dependent chromatin remodeler CHD1L; Amplified in liver cancer protein 1; Chromo domain-containing protein 1-like; CHD1L; ALC1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CHD1L |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18C96 is a rabbit monoclonal antibody that recognizes ATP-dependent chromatin remodeler CHD1L in human and mouse samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 101.0 kDa, and the supplier reports an observed band at about 110 kDa.
CHD1L (ALC1) is an ATP-dependent chromatin remodeler that is recruited to DNA damage sites by poly(ADP-ribose) made by PARP1. By relaxing chromatin at lesions it helps DNA repair proceed, and it is studied in genome stability and in responses to PARP inhibitors.
| Target | ATP-dependent chromatin remodeler CHD1L (gene CHD1L; UniProt Q86WJ1, human) |
|---|---|
| Clone | 18C96 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 101.0 kDa |
| Observed MW | 110 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:40 |
| Flow cytometry | 1:200 |
Samples with a confirmed band (WB): A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CHD1L.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places ATP-dependent chromatin remodeler CHD1L in the nucleus and chromosome. Tissue expression noted by UniProt (human): Frequently overexpressed in hepatomacellular carcinomas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ning-Fang Ma et al. (2008) Isolation and characterization of a novel oncogene, amplified in liver cancer 1, within a commonly amplified region at 1q21 in hepatocellular carcinoma. Hepatology. 10.1002/hep.22072
