| Field | Specification |
|---|---|
| Target | |
| Alternative names | Chromodomain-helicase-DNA-binding protein 4;CHD-4;3.6.4.12;ATP-dependent helicase CHD4;Mi-2 autoantigen 218 kDa protein;Mi2-beta;CHD4; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CHD4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABEI-3 is a rabbit monoclonal antibody that recognizes ATP-dependent chromatin remodeler CHD4 in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 218.0 kDa, and the supplier reports an observed band at about 260 kDa.
CHD4 (Mi-2β) is an ATP-dependent chromatin remodeler and the main catalytic subunit of the NuRD complex, which couples nucleosome remodeling with histone deacetylation. It represses gene expression during development and is recruited to DNA damage sites to support repair, so it is studied in chromatin biology, development and cancer.
| Target | ATP-dependent chromatin remodeler CHD4 (gene CHD4; UniProt Q14839, human) |
|---|---|
| Clone | ABEI-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 218.0 kDa |
| Observed MW | 260 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CHD4.
Reactivity Notes
The supplier lists reactivity with human. UniProt places ATP-dependent chromatin remodeler CHD4 in the nucleus. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
