| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Chromodomain-helicase-DNA-binding protein 4;CHD-4;3.6.4.12;ATP-dependent helicase CHD4;Mi-2 autoantigen 218 kDa protein;Mi2-beta;CHD4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human CHD4 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CHD4 Rabbit Monoclonal Antibody is an antibody targeting CHD4. Common applications include WB, ICC, IF, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: ABEI-3; isotype: Rabbit IgG; reactivity: Human; observed MW: 80 kDa; calculated MW: 218005 MW.
Boster Bio Anti-CHD4 Rabbit Monoclonal Antibody catalog # M01125. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CHD4 — Chromodomain-helicase-DNA-binding protein 4
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: ABEI-3; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 80 kDa; Calculated: 218005 MW
Biological background
Protein function (datasheet): Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. .
Cellular localization (datasheet): Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Associates with centrosomes in interphase. .
Tissue details (datasheet): Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas. .
Research relevance and current trends
- Commonly studied in contexts related to Autoimmune,2339,Chromatin Remodeling,Epigenetics and Nuclear Signaling,Immune System Diseases,Immunology,Transcription.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
