| Field | Specification |
|---|---|
| Target | |
| Alternative names | Choline O-acetyltransferase (EC:2.3.1.6); CHOACTase; ChAT; Choline acetylase; Chat |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived rat Choline Acetyltransferase/Chat recombinant protein (Position: E19-D612). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects choline O-acetyltransferase (gene Chat) in mouse and rat samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 71.9 kDa, and the supplier reports an observed band at about 71 kDa.
Choline acetyltransferase (ChAT) synthesizes the neurotransmitter acetylcholine from choline and acetyl-CoA. It is the defining marker of cholinergic neurons, including motor neurons and basal forebrain neurons, and is widely used in neuroscience research.
| Target | Choline O-acetyltransferase (gene Chat; UniProt P32738, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 71.9 kDa |
| Observed MW | 71 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse spinal cord tissue, rat spinal cord tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant rat Choline Acetyltransferase/Chat fragment (Glu19–Asp612), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhao Dan et al. (2017) Pharmacologic activation of cholinergic alpha7 nicotinic receptors mitigates depressive-like behavior in a mouse model of chronic stress. Journal of Neuroinflammation. 10.1186/s12974-017-1007-2
Pan-Pan Tan et al. (2013) Activation of Muscarinic Receptors Protects against Retinal Neurons Damage and Optic Nerve Degeneration In Vitro and In Vivo Models. CNS Neuroscience & Therapeutics. 10.1111/cns.12187
Yu Huangfei et al. (2017) Acetylcholine acts through M3 muscarinic receptor to activate the EGFR signaling and promotes gastric cancer cell proliferation. Scientific Reports. 10.1038/srep40802
Yanqun Wu et al. (2010) Induced Multilineage Differentiation of Chicken Embryonic Germ Cells via Embryoid Body Formation. Stem Cells and Development. 10.1089/scd.2008.0383
Dogan Unlu Melike et al. (2024) Lercanidipine ameliorated doxorubicin-induced neuroinflammation and maintained the expressions of choline acetyltransferase via enhancing the levels of PI3K/AKT/HIF1-α expressions. Molecular Biology Reports. 10.1007/s11033-023-09024-w
Rui-Xia Xu et al. (2009) PROTECTIVE EFFECTS OF ICARIIN ON COGNITIVE DEFICITS INDUCED BY CHRONIC CEREBRAL HYPOPERFUSION IN RATS. Clinical and Experimental Pharmacology and Physiology. 10.1111/j.1440-1681.2009.05149.x
Hu Rui et al. (2016) Autonomic remodeling may be responsible for decreased incidence of aortic dissection in STZ-induced diabetic rats via down-regulation of matrix metalloprotease 2. BMC Cardiovascular Disorders. 10.1186/s12872-016-0375-3
Limin Wang et al. (2015) Overexpression of NTRK1 Promotes Differentiation of Neural Stem Cells into Cholinergic Neurons. Biomed Research International. 10.1155/2015/857202
Cheng Shigang et al. (2009) Culture of motor neurons from newborn rat spinal cord. Journal of Huazhong University of Science and Technology-Medical Sciences. 10.1007/s11596-009-0404-x
Jun-Qing Yang et al. (2008) Protection of Mouse Brain from Aluminum-induced Damage by Caffeic Acid. CNS Neuroscience & Therapeutics. 10.1111/j.1527-3458.2007.00031.x
