| Field | Specification |
|---|---|
| Target | |
| Alternative names | Choline O-acetyltransferase; CHOACTase; ChAT; Choline acetylase |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Choline Acetyltransferase |
| Molecular weight | |
| Purification | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 31C75 is a rabbit monoclonal antibody that recognizes choline O-acetyltransferase (gene CHAT) in mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 82.5 kDa, and the supplier reports an observed band at about 72 kDa.
Choline acetyltransferase (ChAT) synthesizes the neurotransmitter acetylcholine from choline and acetyl-CoA. It is the defining marker of cholinergic neurons, including motor neurons and basal forebrain neurons, and is widely used in neuroscience research.
| Target | Choline O-acetyltransferase (gene CHAT; UniProt P28329, human) |
|---|---|
| Clone | 31C75 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 82.5 kDa |
| Observed MW | 72 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human choline Acetyltransferase.
Reactivity Notes
The supplier lists reactivity with mouse and rat.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
