| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neuronal acetylcholine receptor subunit alpha-5; CHRNA5; NACHRA5 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CHRNA5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24C16 is a rabbit monoclonal antibody that recognizes neuronal acetylcholine receptor subunit alpha-5 (gene CHRNA5) in human, mouse and rat samples and is validated for Western blot, immunoprecipitation and flow cytometry. The predicted molecular weight is 53.1 kDa, and the supplier reports an observed band at about 55 kDa.
The neuronal acetylcholine receptor alpha-5 subunit (CHRNA5) is an accessory subunit that joins other alpha and beta subunits in pentameric nicotinic receptors, modifying their calcium permeability and sensitivity. Genetic variants in CHRNA5 are among the best-established risk factors for nicotine dependence.
| Target | Neuronal acetylcholine receptor subunit alpha-5 (gene CHRNA5; UniProt P30532, human) |
|---|---|
| Clone | 24C16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 53.1 kDa |
| Observed MW | 55 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CHRNA5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places neuronal acetylcholine receptor subunit alpha-5 in the synaptic cell membrane and cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yu Su et al. (2021) Transgenic expression of SOD1 specifically in neurons of Sod1 deficient mice prevents defects in muscle mitochondrial function and calcium handling. Free Radical Biology and Medicine. 10.1016/j.freeradbiomed.2021.01.047
