| Field | Specification |
|---|---|
| Target | |
| Alternative names | Visual system homeobox 2;Ceh-10 homeodomain-containing homolog;Homeobox protein CHX10;VSX2;CHX10, HOX10; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human CHX10 recombinant protein (Position: A249-A361). Human CHX10 shares 90.3% amino acid (aa) sequence identity with mouse CHX10. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes visual system homeobox 2 (gene VSX2) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 39.4 kDa, and the supplier reports an observed band at about 43 kDa.
VSX2 (CHX10) is a homeodomain transcription factor that is essential for development of the neural retina. It is expressed in retinal progenitor cells and later in bipolar cells, and its loss causes microphthalmia. VSX2 also marks V2a interneurons in the spinal cord and is studied in eye and neural development.
| Target | Visual system homeobox 2 (gene VSX2; UniProt P58304, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 39.4 kDa |
| Observed MW | 43 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Human) |
|---|
Samples with a confirmed band (WB): mouse eye ball tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human CHX10 fragment (Ala249–Ala361), expressed in E. coli. Sequence identity with the mouse ortholog: 90.3%.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places visual system homeobox 2 in the nucleus. Tissue expression noted by UniProt (human): Abundantly expressed in retinal neuroblasts during eye development and in the inner nuclear layer of the adult retina. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Huang Xiao Yong et al. (2008) Characteristics of retinal stem cells from rat optic cup at embryonic day 12.5 (tailbud stage). Cell and Tissue Research. 10.1007/s00441-008-0653-1
