Anti-CITED1 Antibody Picoband®

Overview

Anti-CITED1 Antibody Picoband® is an antibody for CITED1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: CITED1 (vasoactive intestinal peptide receptor 1); UniProt: Q99966
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 23 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-CITED1 Antibody Picoband® catalog # A04351-1.

Biological background

Biological context: PACAP-27 > PACAP-38.

Expression and localization notes: cellular localization: Cell membrane; Multi-pass membrane protein., tissue context: In lung, HT-29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and SUP-T1, but not in the T- cell lines Peer, MOLT-4, HSB and YT..

Common research applications

• Western blotting (WB): Compare CITED1 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of CITED1 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify CITED1-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Cbp/p300-interacting transactivator 1 is a protein that in humans is encoded by the CITED1 gene. This gene encodes a member of the CREB-binding protein/p300-interacting transactivator with Asp/Glu-rich C-terminal domain (CITED) family of proteins. The encoded protein, also known as melanocyte-specific gene 1, may function as a transcriptional coactivator and may play a role in pigmentation of melanocytes. Alternatively spliced transcript variants have been described.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane; Multi-pass membrane protein.
• Tissue details: In lung, HT-29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and SUP-T1, but not in the T- cell lines Peer, MOLT-4, HSB and YT.
• Research category: Adaptive Immunity,B Cells,Hematopoietic Progenitors,Immunoglobulins,Immunology,Lymphoid,Receptors,Stem Cells

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.