| Field | Specification |
|---|---|
| Target | |
| Alternative names | Citrate synthase, mitochondrial; Citrate (Si)-synthase; CS |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Citrate synthetase Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACAO-3 is a rabbit monoclonal antibody that recognizes citrate synthase, mitochondrial (gene CS) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 51.7 kDa, and the supplier reports an observed band at about 45 kDa.
Citrate synthase (CS) is the mitochondrial matrix enzyme that carries out the first step of the tricarboxylic acid (Krebs) cycle, joining acetyl-CoA with oxaloacetate to form citrate. Because its activity tracks mitochondrial content, it is widely used as a marker of mitochondrial mass in studies of energy metabolism, exercise physiology and metabolic disease.
| Target | Citrate synthase, mitochondrial (gene CS; UniProt O75390, human) |
|---|---|
| Clone | ACAO-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 51.7 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells, mouse spleen.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human citrate synthetase Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places citrate synthase, mitochondrial in the mitochondrion matrix. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhang Zijuan et al. (2025) Spatiotemporally resolved mapping of extracellular proteomes via in vivo-compatible TyroID. Nature Communications. 10.1038/s41467-025-57767-w
Zhiduan Cai et al. (2023) Aberrant Expression of Citrate Synthase is Linked to Disease Progression and Clinical Outcome in Prostate Cancer. Cancer Management and Research. 10.2147/CMAR.S255817
